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The gene sequences of WSN influenza virus pressure used on this research have been deposited in GenBank beneath accession numbers CY034138.1, CY034139.1, CY034135.1, CY034134.1, X17336.1, HE802059.1, L25818.1 and CY034136.1. Three-dimensional constructions of HA have been deposited within the Protein Knowledge Financial institution (PDB) with PDB ID 1RVT. All requests for uncooked and analyzed knowledge and supplies are promptly reviewed by our establishment to confirm whether or not the request is topic to any mental property or confidentiality obligations. Any knowledge and supplies that may be shared will likely be launched by way of a cloth switch settlement. All different knowledge that help the findings of this research will likely be offered by the corresponding writer upon cheap request when potential. All knowledge generated or analyzed throughout this research are included within the printed article and its Supplementary Information information. Source data are supplied with this paper.
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Acknowledgements
We acknowledge the Division of Laboratory Animal Science at Peking College Well being Science Middle for help with animal experiments. We acknowledge Sangon Biotech Ltd., GeneWiz lnc. and High Peptide Inc. for gene sequencing, plasmid building and peptide synthesis. We thank B. Xu and related workers from the State Key Laboratory of Pure and Biomimetic Medicine for help of technical and experimental platforms. We thank C. Gong (Sichuan College) for gifting the B16-F10-OVA cell line and Y. Wang (Peking College) for gifting the 4T1-luci cell line. We thank Z. Jiang (Peking College) for help with stream cytometry expertise; L. Zhang (Peking College) for help with transmission electron microscopy; and X. Yan (Xiamen College) for help with nanoflow work. This work was supported by the Nationwide Pure Science Basis of China (grant no. 82130100 to D.Z., grant no. 81821004 to D.Z., grant no. 2017ZX09309009 to D.Z., grant no. 91753202 D.Z. and grant no. 82204258 to D.J.), the Ningbo Key Science and Know-how Improvement Program (grant no. 2022Z136 to D.Z), the Ningbo Yongjiang Expertise Introduction Program (grant no. 2022A-157-G to D.J.) and the China Postdoctoral Science Basis (grant no. 2019M650394 to D.J. and grant no. BX20180017 to D.J.).
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D.Z., D.J. and Y.Z. are inventors on related patent functions held by the Ningbo Institute of Marine Medication, Peking College. The remaining authors declare no competing monetary pursuits.
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Ji, D., Zhang, Y., Solar, J. et al. An engineered influenza virus to ship antigens for lung most cancers vaccination.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01796-7
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DOI: https://doi.org/10.1038/s41587-023-01796-7
